2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. ATP dipotassium

ATP dipotassium  (Synonyms: Adenosine 5'-triphosphate dipotassium)

Cat. No.: HY-B2176C Purity: 98.69%
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ATP dipotassium (Adenosine 5'-triphosphate dipotassium) is a central component of energy storage and metabolism in vivo. ATP dipotassium provides the metabolic energy to drive metabolic pumps and serves as a coenzyme in cells. ATP dipotassium is an important endogenous signaling molecule in immunity and inflammation.

For research use only. We do not sell to patients.

ATP dipotassium Chemical Structure

ATP dipotassium Chemical Structure

CAS No. : 42373-41-1

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Based on 88 publication(s) in Google Scholar

Other Forms of ATP dipotassium:

ATP dipotassium Related Antibodies

Top Publications Citing Use of Products

82 Publications Citing Use of MCE ATP dipotassium

WB
Cell Viability Assay
RT-PCR

    ATP dipotassium purchased from MedChemExpress. Usage Cited in: Immunity. 2024 Mar 12;57(3):495-512.e11.  [Abstract]

    Representative western blots and analyses of the following proteins in BV2 cells treated with vehicle (Con), LPS (200 ng/ml, 3 h), LPS + ATP (3 mM, 30 min), or LPS + ATP-siNKAα1 (one experiment, n = 3). Supernatant (Sup) IL-1β (P17) and cellular lysates (Lys) pro-IL-1β; (I) supernatant caspase-1 (P20) and lysates pro-caspase-1.

    ATP dipotassium purchased from MedChemExpress. Usage Cited in: Transl Res. 2022.

    LPS/ATP treatment significantly promotes expression of NLRP3 and production of N-GSDMD in HAEC. HAEC are pretreated with LPS (500ng/ml) for 5 h and then stimulated with ATP (5 mM) for 1 h.

    ATP dipotassium purchased from MedChemExpress. Usage Cited in: Crit Care. 2021 Oct 12;25(1):356.  [Abstract]

    Stimulation of Raw264.7 macrophages with PBS/PBS-Exo/TNF-Exo for 24 h and treatment with PBS, 5 mM ATP or 20 mM nigericin for 2 h. Cleavage of GSDMD by western blot. GSDMD-FL: full-length GSDMD, GSDMD-N: N-terminal cleavage products of GSDMD.

    ATP dipotassium purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2021 Sep 11.  [Abstract]

    Primary BMDM cells were isolated from WT and Trim21-/- C57BL/6 mice. Cells were primed with 1 μg/mL LPS for 2 h followed by 5 mM ATP or 20 μM Nig or 30 μM flagellin for 30 min. Cell viability are determined by the Cell Counting Kit-8.

    ATP dipotassium purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Feb 18;11(2):132.   [Abstract]

    KCs and BMMs were stimulated with 100 ng/mL LPS for 4 hours and subsequently treated them with 5 mM ATP or 100 ng/mL for 2 hours to induce pyroptosis. The RNA level of lnc-Lfar1 was determined by qRT-PCR.

    ATP dipotassium purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Feb 18;11(2):132.   [Abstract]

    KCs were transfected with lnc-Lfar1 siRNA for 24 h, followed by stimulation with 100 ng/ml LPS for 4 h and subsequently treated with 5 mM ATP or 100 ng/ml for 2 h to induce pyroptosis. a, b The protein level of NLRP3, Caspase1 and GSDMD was determined by western blot. GAPDH was used as an internal control.

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    Description

    ATP dipotassium (Adenosine 5'-triphosphate dipotassium) is a central component of energy storage and metabolism in vivo. ATP dipotassium provides the metabolic energy to drive metabolic pumps and serves as a coenzyme in cells. ATP dipotassium is an important endogenous signaling molecule in immunity and inflammation[1][2].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    ATP dipotassium (5 mM; 1 hour) co-treatment with LPS (1 μg/mL) has a synergistic effect on the activation of the NLRP3 inflammasome in HGFs[3].
    ATP dipotassium (2 mM; 0.5-24 hours) induces secretion of IL-1β, KC and MIP-2 from BMDMs in a caspase-1 activation-dependent manner[4].
    ATP dipotassium promotes neutrophil chemotaxis in vitro[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ATP dipotassium Related Antibodies
    In Vivo

    ATP dipotassium (50 mg/kg; i.p.) protects mice against bacterial infection in vivo[4].
    ATP dipotassium induces the secretion of IL-1β, KC and MIP-2 and neutrophils recruitment in vivo[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Four-week-old Kunming mice (18-22 g)[4]
    Dosage: 50 mg/kg
    Administration: Intraperitoneal injection, before bacterial (E. coli) challenge
    Result: Protected mice from bacterial infection.
    Molecular Weight

    585.38

    Formula

    C10H16K2N5O13P3
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=P(O)(O)OP(OP(OC[C@H]1O[C@@H](N2C3=NC=NC(N)=C3N=C2)[C@H](O)[C@@H]1O)(O)=O)(O)=O.[K].[K]
    Structure Classification
    Initial Source

    widespread
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 70 mg/mL (119.58 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7083 mL 8.5415 mL 17.0829 mL
    5 mM 0.3417 mL 1.7083 mL 3.4166 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 1.7083 mL 8.5415 mL 17.0829 mL 42.7073 mL
    5 mM 0.3417 mL 1.7083 mL 3.4166 mL 8.5415 mL
    10 mM 0.1708 mL 0.8541 mL 1.7083 mL 4.2707 mL
    15 mM 0.1139 mL 0.5694 mL 1.1389 mL 2.8472 mL
    20 mM 0.0854 mL 0.4271 mL 0.8541 mL 2.1354 mL
    25 mM 0.0683 mL 0.3417 mL 0.6833 mL 1.7083 mL
    30 mM 0.0569 mL 0.2847 mL 0.5694 mL 1.4236 mL
    40 mM 0.0427 mL 0.2135 mL 0.4271 mL 1.0677 mL
    50 mM 0.0342 mL 0.1708 mL 0.3417 mL 0.8541 mL
    60 mM 0.0285 mL 0.1424 mL 0.2847 mL 0.7118 mL
    80 mM 0.0214 mL 0.1068 mL 0.2135 mL 0.5338 mL
    100 mM 0.0171 mL 0.0854 mL 0.1708 mL 0.4271 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    ATP dipotassium Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ATP dipotassium42373-41-1Adenosine 5'-triphosphate dipotassiumEndogenous MetaboliteenergystoragemetabolismimmunityinflammationNLRP3inflammasomecytokineInhibitorinhibitorinhibit

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