Apyrase 

Apyrase is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

Product Information
Apyrase requires divalent metal ions for activity,with optimal activity observed with Ca at 5 mM.For hydrolysis of organic di-and triphosphates,the optimal pH is 6.For inorganic substrates,the optimal pH is 5.1.

Instructions
This product is soluble in water (1 mg/mL).The concentration of the enzyme solution in water should be greater than 1mg/mL. It can be stored stably by freezing and dividing it into equal parts. Buffer solutions with a pH value between 5 and 7.5 can be used as solvents for cryogenic equal division storage. For enzyme solutions <1mg/mL, dissolve them in HEPES buffer (pH 7.5), which contains 1mM MgC1₂, 1mM DTT, 1mM EDTA and 1mg/mL bovine albumin.
Repeated freeze-thaw cycles and exposure to room temperature for several hours will cause the enzyme to lose its activity. When Apyrase solution is stored at 0-4°C, it will gradually form a black insoluble precipitate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9000-95-7

Solid

Gray to brown

[Apyrase]

3.6.1.5

≥ 3 U/mg protein

One unit is defined as the amount of enzyme that will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30°C.

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.