2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species (ROS)
  4. APF

APF is a fluorescence probe that can selectively, and dose dependently detect certain species among ROS and that are highly resistant to autoxidation. APF can be used in enzymatic and cellular systems.

For research use only. We do not sell to patients.

APF

APF Chemical Structure

CAS No. : 359010-70-1

Size Stock
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 Check price and availability
Solution
10 mM * 1 mL in DMSO Check price and availability
Solid
500 μg Check price and availability
1 mg Check price and availability
5 mg Check price and availability
10 mg Check price and availability

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This product is a controlled substance and not for sale in your territory.

APF Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE APF

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

APF is a fluorescence probe that can selectively, and dose dependently detect certain species among ROS and that are highly resistant to autoxidation. APF can be used in enzymatic and cellular systems[1].

Clinical Trial
Molecular Weight

423.42

Formula

C26H17NO5
CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

O=C1OC2(C3=CC=C(OC4=CC=C(N)C=C4)C=C3OC5=CC(O)=CC=C25)C6=CC=CC=C16
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (23.62 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3617 mL 11.8086 mL 23.6172 mL
5 mM 0.4723 mL 2.3617 mL 4.7234 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥99.0%

SDS (393 KB)

COA (251 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3617 mL 11.8086 mL 23.6172 mL 59.0430 mL
5 mM 0.4723 mL 2.3617 mL 4.7234 mL 11.8086 mL
10 mM 0.2362 mL 1.1809 mL 2.3617 mL 5.9043 mL
15 mM 0.1574 mL 0.7872 mL 1.5745 mL 3.9362 mL
20 mM 0.1181 mL 0.5904 mL 1.1809 mL 2.9522 mL
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APF Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

APF359010-70-1Reactive Oxygen Species (ROS)fluorescence probeROSenzymaticcellular systemsInhibitorinhibitorinhibit

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