ZAP-70 Antibody (YA5842)

ZAP-70 Antibody (YA5842) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ZAP-70. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

ZAP70 is a tyrosine kinase essential for regulating adaptive immune responses. It controls motility, adhesion, and cytokine expression in mature T-cells, thymocyte development, and contributes to primary B-lymphocyte activation. Upon TCR stimulation by APCs, phosphorylation cascades recruit ZAP70 to doubly phosphorylated CD247/CD3Z via ITAM motifs, localizing it to activated TCR and releasing its autoinhibited conformation—further stabilized by LCK-mediated phosphorylation. ZAP70 then phosphorylates adapter proteins LAT and LCP2, initiating signaling cascades that drive lymphokine production, T-cell proliferation, and differentiation. It also modulates T-cell cytoskeleton dynamics for effector delivery to APCs and regulates TCR internalization/degradation via interactions with CBL, SLA, and SLA2, thereby controlling TCR surface expression for activation modulation. During thymocyte development, ZAP70 promotes survival and cell-cycle progression in CD4/CD8 double-negative stages. By similar mechanisms, it may support primary B-cell formation/activation through BCR signaling.

P43403

Predicted band size: 70 kDa; Observed band size: 70 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

IHC: 1:200-1:1000; WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG