ZAP-70 Antibody (YA5284)

ZAP-70 Antibody (YA5284) is a mouse-derived and non-conjugated monoclonal antibody, targeting to ZAP-70. It can be applicated for WB, IP assays, in the background of human.

ZAP70 is a tyrosine kinase essential for regulating adaptive immune responses. It controls motility, adhesion, and cytokine expression in mature T-cells, thymocyte development, and contributes to primary B-lymphocyte activation. Upon TCR stimulation by APCs, phosphorylation cascades recruit ZAP70 to doubly phosphorylated CD247/CD3Z via ITAM motifs, localizing it to activated TCR and releasing its autoinhibited conformation—further stabilized by LCK-mediated phosphorylation. ZAP70 then phosphorylates adapter proteins LAT and LCP2, initiating signaling cascades that drive lymphokine production, T-cell proliferation, and differentiation. It also modulates T-cell cytoskeleton dynamics for effector delivery to APCs and regulates TCR internalization/degradation via interactions with CBL, SLA, and SLA2, thereby controlling TCR surface expression for activation modulation. During thymocyte development, ZAP70 promotes survival and cell-cycle progression in CD4/CD8 double-negative stages. By similar mechanisms, it may support primary B-cell formation/activation through BCR signaling.

P43403

Observed band size: 70 kDa

affinity chromatography.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:1000

WB, IP

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant human ZAP-70 protein fragments expressed in E.coli.