YB1 Antibody (YA004)

YB1 Antibody (YA004) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to YB1.

YB1 is a DNA- and RNA-binding protein involved in various biological processes, including translational repression, RNA stabilization, mRNA splicing, DNA repair, and transcription regulation. It primarily functions as an RNA-binding protein, preferentially binding to the 5'-[CU]CUGCG-3' RNA motif and specifically recognizing mRNA transcripts modified by C5-methylcytosine (m5C). YB1 promotes mRNA stabilization by binding to m5C-containing mRNAs and recruiting the mRNA stability maintainer ELAVL1, thereby preventing mRNA decay. It is a component of the CRD-mediated complex that enhances MYC mRNA stability. Additionally, YB1 contributes to translation regulation by modulating interactions between mRNA and eukaryotic initiation factors (by similar). It plays a key role in defining the RNA composition of extracellular exosomes by sorting small non-coding RNAs (e.g., tRNAs, Y RNAs, Vault RNAs, and miRNAs) through recognition of m5C-containing RNAs. YB1 also acts as a key effector in epidermal progenitors, preventing senescence by regulating the translation of senescence-associated cytokine mRNAs. It is involved in pre-mRNA alternative splicing regulation by binding to splice sites. Furthermore, YB1 binds DNA, enhancing transcription of the multidrug resistance gene MDR1 in the presence of acetylated APEX1. It exhibits endonucleolytic activity, introducing nicks or breaks into double-stranded DNA, suggesting a role in DNA repair. The secreted form of YB1 functions as an extracellular mitogen, stimulating cell migration and proliferation.

Free

P67809

Predicted band size: 36 kDa; Observed band size: 50 kDa

Protein A affinity purified.

Cytoplasm, Nucleus, Cytoplasmic granule, Secreted.

Non-conjugated

Unmodified

AB_3102464

Tags & Cell Markers

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1,000-1:5,000; ICC/IF: 1:100-1:500; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from Hela (lane 1) and 293T (lane 2) and RAW264.7 (lane 3) using YB1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ICC/IF, IHC-P, FC, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human YB1.AA range:275-324.