Wnt3a Antibody (YA5319)

Wnt3a Antibody (YA5319) is a mouse-derived and non-conjugated monoclonal antibody, targeting to Wnt3a. It can be applicated for WB assays, in the background of human.

The Wnt3a protein serves as a ligand for members of the frizzled family of seven transmembrane receptors, likely functioning in the canonical Wnt signaling pathway, resulting in the activation of transcription factors of the TCF/LEF family. It is essential for normal embryonic mesoderm development, caudal somite formation, and morphogenesis of the developing neural tube. Wnt3a also plays a role in mediating the self-renewal of stem cells at the base of intestinal crypts in vitro. The protein forms a soluble 1:1 complex with AFM, preventing oligomerization and ensuring prolonged biological activity; this complex may represent the physiological form in body fluids. Wnt3a forms homooligomers through disulfide linkages, which lead to inactivation. It interacts with PORCN and components of the Wnt-Fzd-LRP5-LRP6 signaling complex, including LRP6, APCDD1, and WLS. Additionally, Wnt3a interacts with glypican GPC3 and PKD1 via its extracellular domain. These interactions underscore the complexity of Wnt3a's involvement in various developmental and signaling processes.

P56704

Observed band size: 42 kDa

affinity chromatography.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:1000

WB

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant Wnt3a protein fragments expressed in E.coli.