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  4. Monoclonal Antibodies Recombinant Antibodies
  5. VPS35 Antibody (YA3314)

VPS35 Antibody (YA3314)

Cat. No.: HY-P83569
COA
SDS
User Guide for Antibodies Technical Support

VPS35 Antibody (YA3314) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3314), targeting VPS35, with a predicted molecular weight of 92 kDa (observed band size: 81 kDa). VPS35 Antibody (YA3314) can be used for WB, IHC-P experiment in human, mouse, rat background.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

VPS35 Antibody (YA3314) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3314), targeting VPS35, with a predicted molecular weight of 92 kDa (observed band size: 81 kDa). VPS35 Antibody (YA3314) can be used for WB, IHC-P experiment in human, mouse, rat background.
Background

VPS35 is a component of the retromer cargo-selective complex (CSC), which serves as the core functional unit of retromer or its variants to prevent mis-sorting of specific transmembrane cargo proteins into the lysosomal degradation pathway. The CSC is recruited to endosomal membranes via RAB7A and SNX3. VPS35 primarily mediates cargo selectivity by interacting with the cytoplasmic domains of cargo proteins, though these interactions exhibit low affinity, and retromer SNX proteins may also contribute to cargo selection, challenging the classical CSC model. The SNX-BAR retromer facilitates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and participates in endosome-to-plasma membrane recycling. The SNX3-retromer mediates WLS transport from endosomes to the TGN via a distinct pathway from the SNX-BAR retromer. The SNX27-retromer is involved in endosome-to-plasma membrane trafficking and recycling of diverse cargo proteins. The CSC acts as a hub for recruiting other proteins, such as the WASH complex and TBC1D5. VPS35 is essential for retrograde transport of lysosomal enzyme receptors IGF2R and SLC11A2, regulates transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA), and is required for endosomal localization of WASHC2C and TBC1D5. It also mediates CSC association with the WASH complex via WASHC2. During microbial infection, the heterotrimeric retromer CSC facilitates human papillomavirus exit from early endosomes and delivery to the Golgi apparatus.
Gene ID
SwissProt ID
Synonyms
hVPS35; MEM3; PARK17; VPS35
Molecular Weight

Predicted band size: 92 kDa; Observed band size: 81 kDa
Purity

Affinity Purified
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Signal Transduction
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse, Rat
Dilution Ratio

WB: 1/500-1/1000;IHC: 1/50-1/100
Application

WB, IHC-P
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

A synthetic peptide of human VPS35
Database

Entrez Gene: 100133770 Human ; 55737 Human ; 65114 Mouse ; 25479 Rat

SwissProt: Q96QK1 Human ; Q9EQH3 Mouse ;

OMIM: 614203 Human

Documentation
SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

VPS35 Antibody (YA3314) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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VPS35 Antibody (YA3314)
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