Villin Antibody (YA5659)

Villin Antibody (YA5659) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Villin.

VIL1 is an epithelial cell-specific Ca²⁺-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. It plays roles in actin nucleation, actin filament bundle assembly, actin filament capping, and severing. This protein binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA) with higher affinity for LPA. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities, while binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling. VIL1 regulates intestinal epithelial cell morphology, invasion, migration, and apoptosis, protecting against dextran sodium sulfate (DSS)-induced apoptosis in the gastrointestinal epithelium, likely by maintaining mitochondrial integrity. It also enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis, and wound repair. During S.flexneri infection, its actin-severing activity promotes actin-based bacterial motility and dissemination.

P09327

Predicted band size: 93 kDa; Observed band size: 93 kDa

Protein G

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Pig, Bovin,

IHC: 1:100-500; WB: 1:500-2000; ICC/IF: 1:100-500; ELISA: 1:1000-5000

IHC-P, WB, ICC/IF, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Synthesized peptide derived from human Villin AA range: 150-250