Villin Antibody (YA3724)

Villin Antibody (YA3724) is a mouse-derived and non-conjugated IgG1 antibody, targeting to VIL1. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human.

VIL1 is an epithelial cell-specific Ca²⁺-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. It plays roles in actin nucleation, actin filament bundle assembly, actin filament capping, and severing. This protein binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA) with higher affinity for LPA. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities, while binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling. VIL1 regulates intestinal epithelial cell morphology, invasion, migration, and apoptosis, protecting against dextran sodium sulfate (DSS)-induced apoptosis in the gastrointestinal epithelium, likely by maintaining mitochondrial integrity. It also enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis, and wound repair. During S.flexneri infection, its actin-severing activity promotes actin-based bacterial motility and dissemination.

P09327

Predicted band size: 93 kDa; Observed band size: 93 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

WB: 1/500-1/2000; IHC: 1/200-1/1000; IF: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human VIL1 (AA: 1-209) expressed in E. Coli.