VDAC1 Antibody (YA6199)

VDAC1 Antibody (YA6199) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VDAC1. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

VDAC1 protein serves as a versatile channel, permeating both the mitochondrial outer membrane and the plasma membrane. This dual functionality is pivotal, as the channel in the outer mitochondrial membrane facilitates the diffusion of small hydrophilic molecules, while in the plasma membrane, it plays a role in cell volume regulation and apoptosis. VDAC1 exhibits a dynamic conformational switch, adopting an open state at low or zero membrane potential and a closed state at potentials above 30-40 mV. The open state displays weak anion selectivity, whereas the closed state exhibits cation selectivity. Notably, VDAC1 binds various signaling molecules, including the sphingolipid ceramide, phospholipid phosphatidylcholine, and sterol cholesterol. In depolarized mitochondria, VDAC1 acts downstream of PRKN and PINK1, participating in mitophagy promotion or apoptosis prevention. Polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx, ultimately inhibiting apoptosis. Additionally, VDAC1 may contribute to the formation of the permeability transition pore complex (PTPC), which releases mitochondrial products triggering apoptosis. Furthermore, it has been implicated in mediating ATP export from cells and is subject to inhibition by nitric oxide. The multifaceted functions of VDAC1 underscore its significance in various cellular processes, ranging from ion transport to cellular homeostasis and apoptosis regulation.

P21796

Predicted band size: 31 kDa; Observed band size: 33 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

IHC: 1:200-1:1000; WB: 1:1000-1:5000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200,

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG