Ubiquitin Antibody (YA5386)

Ubiquitin Antibody (YA5386) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Ubiquitin.

UBB protein, a pivotal player in cellular regulation, exists either covalently attached to other proteins or in a free, unanchored state. In its covalently bound forms, UBB conjugates to target proteins through isopeptide bonds, presenting as monoubiquitin, polyubiquitin chains linked via different Lys residues, or linear polyubiquitin chains initiated at the Met residue. The functions of polyubiquitin chains are diverse and dependent on the specific Lys residue involved: Lys-6-linked ubiquitin may contribute to DNA repair, Lys-11-linked is implicated in endoplasmic reticulum-associated degradation (ERAD) and cell-cycle regulation, Lys-29-linked is associated with proteotoxic stress response and cell cycle, Lys-33-linked is engaged in kinase modification, Lys-48-linked is crucial for protein degradation via the proteasome, and Lys-63-linked plays roles in endocytosis, DNA-damage responses, and NF-kappa-B activation. Linear polyubiquitin chains, initiated at Met, are linked to cell signaling. While UBB typically conjugates to Lys residues, rare instances involve conjugation to Cys or Ser residues. In its unanchored state, free polyubiquitin contributes to distinct roles, such as activating protein kinases and participating in signaling processes. Furthermore, UBB interacts with SKP1-KMD2A and SKP1-KMD2B complexes, emphasizing its involvement in specific cellular pathways and protein interactions.

P62979;P62987;P62988

affinity chromatography.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Species independent

IHC1:50-200; WB: 1:1000-2000

IHC-P, WB

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Synthesized peptide derived from Ubiquitin