U2AF2 Antibody (YA2221)

U2AF2 Antibody (YA2221) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to U2AF2.

U2AF2 plays a role in pre-mRNA splicing and 3'-end processing. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD) and binding to pre-mRNA, it may couple transcription with splicing. In muscle tissue, it induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion and regulates TNNT2 exon 5 inclusion through competition with MBNL1. During spliceosome assembly, it preferentially binds to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4. It is also required for mRNA nuclear export, even for intron-less genes. Additionally, it represses the splicing of MAPT/Tau exon 10 and positively regulates pre-mRNA 3'-end processing by recruiting the CFIm complex to cleavage and polyadenylation signals.

P26368

Predicted band size: 54 kDa; Observed band size: 60-65 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3104584

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200

• 
  Immunohistochemical analysis of paraffin-embedded mouse liver tissue using U2AF2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse liver tissue using U2AF2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF

Liquid

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human U2AF65