Tuberin Antibody (YA5871)

Tuberin Antibody (YA5871) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Tuberin. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

Tuberin is the catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor stimulating anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. Within the TSC-TBC complex, Tuberin functions as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of mTORC1's protein kinase activity. Under nutrient deprivation, the TSC-TBC complex inhibits mTORC1, preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by mTORC1 signaling. Upon nutrient availability, the TSC-TBC complex is inactivated, relieving mTORC1 inhibition. Additionally, Tuberin participates in microtubule-mediated protein transport by regulating mTORC1 signaling (by similarity) and stimulates the intrinsic GTPase activity of Ras-related proteins RAP1A and RAB5 (by similarity).

P49815

Predicted band size: 201 kDa; Observed band size: 201 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

IHC: 1:200-1:1000; WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG