TRIM25 Antibody (YA4264)

TRIM25 Antibody (YA4264) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to TRIM25.

TRIM25 functions as a ubiquitin E3 ligase and an ISG15 E3 ligase, playing a crucial role in innate immune defense against viruses by mediating the ubiquitination of RIGI and IFIH1. It facilitates 'Lys-63'-linked polyubiquitination of the RIGI N-terminal CARD-like region, potentially contributing to interferon production signaling in response to viral infection. Additionally, TRIM25 mediates 'Lys-63'-linked polyubiquitination of IFIH1 and promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein involved in regulating multiple signaling pathways. It mediates estrogen action in various target organs and regulates ZFHX3 through ubiquitination and subsequent proteasomal degradation. In DNA replication, TRIM25 interacts with the KHDC3L-OOEP scaffold and ubiquitinates BLM to promote the restart of stalled replication forks (by similarity). Furthermore, TRIM25 is essential for the antiviral activity of ZAP/ZC3HAV1, enhancing its binding to target mRNA via 'Lys-63'-linked polyubiquitination. It also ubiquitinates various substrates involved in stress granule formation, nonsense-mediated mRNA decay, nucleoside synthesis, and mRNA translation and stability.

Q14258

Predicted band size: 71 kDa; Observed band size: 71 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal Antibody

Mouse

Human

WB: 1/500-1/2000; IHC: 1/200-1/1000; IF: 1/50-1/250 FC: 1/200-1/400; ELISA: 1/10000

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Purified recombinant fragment of human TRIM25 (AA: 211-360) expressed in E. Coli.