53BP1 Antibody (YA4218)

53BP1 Antibody (YA4218) is a mouse-derived and non-conjugated IgG1 antibody, targeting to TP53BP1. It can be applicated for IHC-P, FC, ELISA assays, in the background of human.

TP53BP1 is a double-strand break (DSB) repair protein involved in DNA damage response, telomere dynamics, and class-switch recombination (CSR) during antibody genesis. It plays a key role in DSB repair by promoting non-homologous end joining (NHEJ)-mediated repair and counteracting the homologous recombination (HR) repair protein BRCA1. Upon DNA damage, phosphorylation by ATM facilitates its interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSB sites. TP53BP1 is recruited to DSBs by recognizing histone H2A monoubiquitinated at Lys-15 (H2AK15Ub) and histone H4 dimethylated at Lys-20 (H4K20me2), which are histone marks present at DSB sites. It is essential for immunoglobulin class-switch recombination (CSR), a process involving DSB generation. Additionally, by similar mechanisms (by similar), TP53BP1 participates in the repair and orientation of broken DNA ends during CSR but is not required for classic NHEJ or V(D)J recombination (by similar). It also promotes NHEJ of dysfunctional telomeres through interaction with PAXIP1.

Q12888

Predicted band size: 214 kDa; Observed band size: 450 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

IHC: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

IHC-P, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human TP53BP1 (AA: 574-773) expressed in E. Coli.