TLR2 Antibody (YA3547)

Cat. No.: HY-P81134: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

TLR2 Antibody (YA3547) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TLR2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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TLR2 Antibody (YA3547) Featured Recommendations:

Publications Citing Use of MCE TLR2 Antibody (YA3547)

•Vet Microbiol. 2025 Sep 11:310:110726. [Abstract]

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

TLR2 Antibody (YA3547) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TLR2.

Host

Rabbit

Clonality

Monoclonal, Recombinant

Molecular Weight

Predicted band size: 90 kDa;

Observed band size: 80-90 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O60603

Gene ID

7097 [NCBI]

Immunogen

Recombinant protein within human TLR2 aa 21-588 (Extracellular).

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
FC FC: Flow Cytometry	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:100

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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ALL WB FC

Western blot analysis of extracts from THP-1(lane 2(20μg) and THP-1(lane 3(40μg) using TLR2(HY-P81134 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Flow cytometric analysis of 1X10^6 THP-1 cells labeling TLR2 Antibody (HY-P81134, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

RRID

AB_3103235

Database

Entrez Gene: 7097 Human ; 24088 Mouse ; 310553 Rat

SwissProt: O60603 Human ; Q9QUN7 Mouse ;

OMIM: 246300 Human

Synonyms

Toll-like receptor 2; Toll like receptor 2; Toll like receptor 2 precursor; Toll-like receptor 2; TLR 2; TLR-2; Toll/interleukin-1 receptor-like protein 4; CD282 antigen; CD282; TIL 4; TIL4; TLR2_HUMAN; Toll/interleukin 1 receptor like 4; Toll/interleukin 1 receptor like protein 4; Toll/interleukin receptor like protein 4.

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (191 KB)

User Guide for Antibodies (1077 KB)

TLR2 Antibody (YA3547) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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TLR2 Antibody (YA3547)

TLR2 Antibody (YA3547) Featured Recommendations:

Publications Citing Use of MCE TLR2 Antibody (YA3547)

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Background

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TLR2 Antibody (YA3547) Related Classifications

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