Tight Junction Protein ZO 3 Antibody (YA2456)

Tight Junction Protein ZO 3 Antibody (YA2456) is a non-conjugated IgG antibody, targeting Tight Junction Protein ZO 3, with a predicted molecular weight of 101 kDa (observed band size: 140 kDa). Tight Junction Protein ZO 3 Antibody (YA2456) can be used for WB experiment in human, rat background.

Tight Junction Protein ZO 3 (TJP3) is a scaffolding protein closely related to TJP1 and TJP2, linking tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. The tight junction functions to limit paracellular movement of substances and serves as a boundary between the distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. TJP3 binds and recruits PATJ to tight junctions, stabilizing apical and lateral components. It also promotes cell-cycle progression by sequestering cyclin D1 (CCND1) at tight junctions during mitosis, preventing its degradation and enabling S-phase transition. Similar to TJP1 and TJP2, TJP3 contributes to the junctional retention and stability of the transcription factor DBPA but is not involved in its nuclear shuttling. Unlike TJP2, TJP3 is dispensable for viability, embryonic development, epithelial differentiation, and TJ establishment, at least under laboratory conditions.

O95049

Predicted band size: 101 kDa; Observed band size: 140 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3104810

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat

WB: 1/500-1/1000

• 
  Western blot analysis of extracts from Caco-2 (lane 2(20μg)) using Zonula occludens protein 3 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human Zonula occludens protein 3