Telomeric Repeat Binding Factor 2 Antibody (YA4288)

Telomeric Repeat Binding Factor 2 Antibody (YA4288) is a mouse-derived and non-conjugated IgG1 antibody, targeting to TERF2. It can be applicated for IHC-P, ELISA assays, in the background of human.

TERF2 is a protein that binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. Beyond its telomeric DNA-binding function, it recruits multiple factors and enzymes essential for telomere protection, including the shelterin complex, TERF2IP/RAP1, and DCLRE1B/Apollo. As a component of the shelterin complex (telosome), TERF2 regulates telomere length and shields chromosome ends from DNA damage surveillance and inappropriate processing by DNA repair pathways. Collaborating with DCLRE1B/Apollo, TERF2 is crucial for telomeric loop (T loop) formation by generating 3' single-stranded overhangs at leading-end telomeres, protecting them from degradation and repair. It also recruits and activates the exonuclease activity of DCLRE1B/Apollo, preferentially binds positive supercoiled DNA, and, together with DCLRE1B/Apollo, controls DNA topoisomerase (TOP1, TOP2A, and TOP2B) levels during telomere replication to prevent aberrant topology. By similarity, TERF2 recruits TERF2IP/RAP1 to telomeres, contributing to the repression of homology-directed repair (HDR), which may influence telomere length.

Q15554

Predicted band size: 60 kDa; Observed band size: 66 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

IHC: 1/200-1/1000; ELISA: 1/10000

IHC-P, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human TERF2 (AA: 324-500) expressed in E. Coli.