Telomeric Repeat Binding Factor 2 Antibody (YA2304)

Telomeric Repeat Binding Factor 2 Antibody (YA2304) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2304), targeting Telomeric Repeat Binding Factor 2, with a predicted molecular weight of 60 kDa (observed band size: 66 kDa). Telomeric Repeat Binding Factor 2 Antibody (YA2304) can be used for WB, IHC-P, ICC/IF experiment in human background.

The related gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. The protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic.

Q15554

Predicted band size: 60 kDa; Observed band size: 66 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3104660

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200

• 
  Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), Daudi (lane 2, 20 μg), 293 (lane 3, 20 μg), and K562 (lane 4, 20 μg) using Telomeric Repeat Binding Factor 2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P, ICC/IF

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human TRF2