STAT5a Antibody (YA054)

Cat. No.: HY-P80904: Data Sheet COA
SDS
User Guide for Antibodies
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STAT5 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 91 kDa, targeting to STAT5. It can be used for WB,ICC/IF,IP assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

Biological Activity
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Description	STAT5 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 91 kDa, targeting to STAT5. It can be used for WB,ICC/IF,IP assays with tag free, in the background of Human, Mouse, Rat.
Background	STAT5: Enables DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and phosphate ion binding activity. Involved in growth hormone receptor signaling pathway via JAK-STAT and regulation of growth. Acts upstream of or within several processes, including animal organ development; positive regulation of leukocyte proliferation; and regulation of hemopoiesis. Located in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; hemolymphoid system; and liver and biliary system. Orthologous to human STAT5A (signal transducer and activator of transcription 5A). [provided by Alliance of Genome Resources, Apr 2022]
Tag	Free
Gene ID	6776 [NCBI]
SwissProt ID	P42230
Synonyms	MGF; STAT5
Molecular Weight	Predicted band size: 91 kDa; Observed band size: 91 kDa
Purity	affinity purified
Conjugation	Non-conjugated
Modification	Unmodified
RRID	AB_3102249
Research Field	Signal Transduction
Product Categories	Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality	Recombinant, Monoclonal
Host	Rabbit
Reactivity	Human, Mouse, Rat
Dilution Ratio	WB: 1:500-1:1,000 ;IF: 1:50-1:200 ;IP: 1:20 Western blot analysis was performed on extracts from PC-12 (lane 1, 20 μg), C6 (lane 2, 20 μg), RAW264.7 (lane 3, 20 μg), 3T3 (lane 4, 20 μg), HeLa (lane 5, 20 μg), and A549 (lane 6, 20 μg) using STAT5 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C. Western blot analysis of extracts from 293T (lane 1) and K562 (lane 2) and HepG2 (lane 3) using STAT5 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
Application	WB, ICC/IF, IP
Appearance	Liquid
Formulation	Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG
Sensitivity	Endogenous
Immunogen	Synthetic peptide corresponding to Mouse STAT5A aa600-786.
Database	Entrez Gene: 6776 Human ; 6777 Human ; 20850 Mouse ; 20851 Mouse ; 24918 Rat 25126 SwissProt: P42229 Human ; P51692 Human ; P42230 Mouse ; P42232 Mouse ; P52632 Rat Q62771 OMIM: 601511 Human
Documentation	Select Batch: Data Sheet (234 KB) SDS (251 KB) COA (191 KB) User Guide for Antibodies (1077 KB)

STAT5a Antibody (YA054) Related Classifications

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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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STAT5a Antibody (YA054)

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