Src Antibody (YA6010)

Src Antibody (YA6010) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Src. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

Src, a non-receptor protein tyrosine kinase, emerges as a central player in an array of cellular processes triggered by the engagement of diverse receptors, including immune response receptors, integrins, receptor protein tyrosine kinases, G protein-coupled receptors, and cytokine receptors. It orchestrates signaling pathways governing gene transcription, immune responses, cell adhesion, cell cycle progression, apoptosis, migration, and cellular transformation. While functional redundancy within the SRC kinase family complicates the identification of specific roles, SRC appears to be a primary kinase activated upon receptor engagement, influencing the activation of other protein tyrosine kinase (PTK) families. SRC is implicated in the regulation of cytoskeletal organization by phosphorylating substrates like AFAP1 and cortactin (CTTN). Its involvement extends to cell-cell and cell-matrix junctions, where it phosphorylates components such as beta-catenin (CTNNB1), delta-catenin (CTNND1), plakoglobin (JUP), and connexin-43 (GJA1). Additionally, SRC participates in PDGF-mediated tyrosine phosphorylation of STAT1 and STAT3, the RAS pathway, EGF-mediated calcium-activated chloride channel activation, and beta-arrestin desensitization. It also plays a crucial role in EGFR internalization, CDK20/MAPK3 mitogen-activated protein kinase cascade stimulation, and osteoclastic bone resorption. SRC's multifaceted activities highlight its intricate role in cellular regulation and signal transduction.

P12931

Predicted band size: 60 kDa; Observed band size: 60 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

IHC: 1:1000-1:4000; WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG