SP1 Antibody (YA064)

SP1 Antibody (YA064) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SP1.

SP1 is a transcription factor capable of activating or repressing transcription in response to physiological and pathological stimuli. It binds with high affinity to GC-rich motifs and regulates the expression of numerous genes involved in processes such as cell growth, apoptosis, differentiation, and immune responses. SP1 is highly regulated by post-translational modifications (e.g., phosphorylation, sumoylation, proteolytic cleavage, glycosylation, and acetylation). It also binds the PDGFR-alpha G-box promoter and may modulate cellular responses to DNA damage while participating in chromatin remodeling. SP1 plays an essential role in regulating FE65 gene expression and, in complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 exhibits stronger transcriptional activation than isoform 1 and positively regulates the core clock component BMAL1. Additionally, SP1 facilitates the recruitment of SMARCA4/BRG1 to the c-FOS promoter. By similarity, SP1 protects cells against oxidative stress following brain injury by regulating RNF112 expression.

Free

P08047

Predicted band size: 81 kDa; Observed band size: 90 kDa

Protein A affinity purified.

Nucleus, Cytoplasm.

Non-conjugated

Unmodified

AB_3102437

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:1000-1:2000; ICC/IF: 1:100-1:500; IHC-P: 1:50-1:200

• 
  Western blot analysis of extracts from Ramos (lane 1) and THP-1 (lane 2) and 293T(lane 3) and HeLa (lane 4) using SP1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ICC/IF, IHC-P

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human SP1.AA range:544-588.