SNAI1 Antibody (YA5064)

SNAI1 Antibody (YA5064) is a mouse-derived and non-conjugated monoclonal antibody, targeting to SNAI1. It can be applicated for WB, ELISA assays, in the background of human.

SNAI1 is a protein involved in the induction of epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, cell survival, and migration. It binds to three E-boxes in the E-cadherin/CDH1 gene promoter and the promoters of CLDN7 and KRT8, recruiting the histone demethylase KDM1A to these promoters, leading to decreased dimethylated H3K4 levels and transcriptional repression. The N-terminal SNAG domain competes with histone H3 for binding to the histone demethylase complex formed by KDM1A and RCOR1, thereby inhibiting demethylation of histone H3 at Lys-4 (in vitro). During EMT, SNAI1 cooperates with LOXL2 to negatively regulate pericentromeric heterochromatin transcription (By similar: SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription, resulting in the release of the heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits). Additionally, SNAI1 associates with EGR1 and SP1 to mediate TPA-induced upregulation of CDKN2B, potentially by binding to the 5'-TCACA-3' sequence in the CDKN2B promoter region, and may also activate the CDKN2B promoter independently.

O95863

Predicted band size: 29 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:2000; ELISA: 1:10000

WB, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of human SNAI 1 expressed in E. Coli.