SNAI1 Antibody (YA3575)

SNAI1 Antibody (YA3575) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SNAI1.

SNAI1 is a protein involved in the induction of epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, cell survival, and migration. It binds to three E-boxes in the E-cadherin/CDH1 gene promoter and the promoters of CLDN7 and KRT8, recruiting the histone demethylase KDM1A to these promoters, leading to decreased dimethylated H3K4 levels and transcriptional repression. The N-terminal SNAG domain competes with histone H3 for binding to the histone demethylase complex formed by KDM1A and RCOR1, thereby inhibiting demethylation of histone H3 at Lys-4 (in vitro). During EMT, SNAI1 cooperates with LOXL2 to negatively regulate pericentromeric heterochromatin transcription (By similar: SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription, resulting in the release of the heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits). Additionally, SNAI1 associates with EGR1 and SP1 to mediate TPA-induced upregulation of CDKN2B, potentially by binding to the 5'-TCACA-3' sequence in the CDKN2B promoter region, and may also activate the CDKN2B promoter independently.

O95863

Predicted band size: 29 kDa; Observed band size: 29 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal Antibody

Mouse

Human

WB: 1:500-1:1000; ELISA: 1:10000

WB, ELISA

Liquid

Supplied in ascitic fluid containing 0.03% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human SNAI1 full(aa 2-264).