SMYD2 Antibody (YA5183)

SMYD2 Antibody (YA5183) is a mouse-derived and non-conjugated monoclonal antibody, targeting to SMYD2. It can be applicated for WB assays, in the background of human, mouse.

SMYD2 protein, a versatile protein-lysine N-methyltransferase, exhibits the capability to methylate both histones and non-histone proteins, such as p53/TP53 and RB1. Notably, it specifically trimethylates histone H3 at 'Lys-4' (H3K4me3) in vivo, a crucial modification associated with gene activation. The enzymatic activity of SMYD2 requires interaction with HSP90alpha, further highlighting its functional dependence on cellular regulatory networks. Strikingly, SMYD2 demonstrates heightened methyltransferase activity on p53/TP53, where it monomethylates 'Lys-370,' leading to a consequential reduction in DNA-binding activity and subsequent transcriptional regulation. Furthermore, SMYD2 plays a role in the epigenetic modification of RB1 by monomethylating 'Lys-860,' showcasing its broad impact on both histone and non-histone proteins, thereby contributing to the intricate landscape of epigenetic regulation within the cellular milieu.

Q9NRG4

Observed band size: 50 kDa

affinity chromatography.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse

WB: 1:1000

WB

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant human KMT3C / SMYD2 protein fragments expressed in E.coli.