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  5. SMURF2 Antibody (YA2372)

SMURF2 Antibody (YA2372)

Cat. No.: HY-P82627
COA
SDS
User Guide for Antibodies Technical Support

SMURF2 Antibody (YA2372) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SMURF2.

For research use only. We do not sell to patients.

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10 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  
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Top Publications Citing Use of Products

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

SMURF2 Antibody (YA2372) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SMURF2.
Background

Enables SMAD binding activity; identical protein binding activity; and ubiquitin-protein transferase activity. Involved in negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of trophoblast cell migration; and ubiquitin-dependent SMAD protein catabolic process. Located in nuclear speck. Part of ubiquitin ligase complex.
Gene ID
SwissProt ID
Synonyms
hSMURF2; SMUF2_HUMAN; Smurf2
Molecular Weight

Predicted band size: 86 kDa; Observed band size: 86 kDa
Purity

Affinity Chromatography
Conjugation

Non-conjugated
Modification

Unmodified
Research Field

Epigenetics and Nuclear Signaling
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse, Rat
Dilution Ratio

WB: 1:500-1:1000

  • Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 15 μg), U2OS (lane 2, 15 μg), HCT116 (lane 3, 15 μg), and Mouse testis (lane 4, 15 μg) using SMURF2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Application

WB
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

A synthesized peptide derived from human SMURF 2
Database

Entrez Gene: 64750 Human ; 66313 Mouse ; 303614 Rat

SwissProt: Q9HAU4 Human ; A2A5Z6 Mouse ;

OMIM: 605532 Human

Documentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

SMURF2 Antibody (YA2372) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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SMURF2 Antibody (YA2372)
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