Smad5 Antibody (YA5909)

Smad5 Antibody (YA5909) is a rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Smad5. It can be applicated for WB, IHC-P, ICC/IF, IP, ELISA assays, in the background of human, mouse, rat.

Smad1/5/9 is a transcriptional regulator involved in various cellular processes such as embryonic development, cell differentiation, angiogenesis, and tissue homeostasis. Upon BMP ligand binding to cell surface receptors, it is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form a heteromeric complex that translocates into the nucleus, functioning as a transcription factor. The hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate signaling network outcomes. Non-phosphorylated SMAD5 plays a cytoplasmic role in energy metabolism regulation by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes. Mechanistically, it interacts with hexokinase 1 (HK1) to accelerate glycolysis.

Q99717

Predicted band size: 55 kDa; Observed band size: 55 kDa

Protein A

Non-conjugated

Unmodified

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal

Rabbit

Human, Mouse, Rat

IHC: 1:200-1:1000; WB: 1:2000-1:10000; ICC/IF: 1:200-1:1000; ELISA: 1:5000-1:20000; IP: 1:50-1:200;

WB, IHC-P, ICC/IF, IP, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG