Smad2 Antibody (YA076)

Smad2 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 52 kDa, targeting to Smad2. It can be used for WB,ICC,IHC-P,IP,FC assays with tag free, in the background of Human, Mouse, Rat.

The SMAD2 protein functions as a receptor-regulated SMAD (R-SMAD), serving as an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. It binds the TRE element in the promoter region of numerous genes regulated by TGF-beta, and upon forming the SMAD2/SMAD4 complex, it activates transcription. SMAD2 plays a role in promoting TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells. Additionally, it positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ, acting as a negative regulator. There is evidence suggesting its potential function as a tumor suppressor in colorectal carcinoma. SMAD2 exists as a monomer in the absence of TGF-beta and forms a heterodimer with co-SMAD, SMAD4, in the nucleus to constitute the transactivation complex SMAD2/SMAD4. It interacts with various proteins, including ZFYVE9, TAZ/WWRT1, FOXH1, SNW1, CREB-binding protein (CBP), EP300, SNON, ALK4/ACVR1B, SKOR1, SKOR2, PRDM16, LEMD3, RBPMS, WWP1, RANBP3, PDPK1, DAB2, USP15, PPP5C, LDLRAD4, PMEPA1, ZFHX3, ZNF451, SMURF2, PPM1A, TGF-beta, TGFBR1, TGIF, TRIM33, ZNF580, NEDD4L, HGS, AIP1, WWP1, PML, ZNF8, RNF111, and YAP1, participating in diverse cellular processes, including signal transduction, transcriptional regulation, and protein-protein interactions.

Free

Q15796

Predicted band size: 52 kDa; Observed band size: 58 kDa

Protein A affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Unmodified

AB_3102428

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

Human, Mouse, Rat

WB: 1:500-1:2,000 ;ICC: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

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  Western blot analysis of extracts from Hela (lane 1) and NIH3T3 (lane 2) and Jurkat (3) using Smad2 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ICC/IF, IHC-P, IP, FC

WB, IHC-P, ICC/IF, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Smad2.AA range:220-270.