Smad1/9 Antibody (YA5567)

Smad1/9 Antibody (YA5567) is a mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Smad1/9. It can be applicated for WB, ICC/IF, ELISA assays, in the background of human, mouse, rat, monkey.

SMAD1, a versatile transcriptional modulator, plays pivotal roles in various cellular processes, including embryonic development, cell differentiation, and tissue homeostasis. Upon binding of BMP ligands to their receptors, SMAD1 undergoes phosphorylation by activated type I BMP receptors, forming a heteromeric complex with SMAD4 that translocates into the nucleus, functioning as a transcription factor. This hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs), thus modulating the signaling network's outcome. Notably, the SMAD1/OAZ1/PSMB4 complex participates in the degradation of the CREBBP/EP300 repressor SNIP1 and positively regulates BMP4-induced expression of the odontogenic development regulator MSX1. Interactions with various partners such as SMAD4, YY1, HGS, NANOG, ZCCHC12, PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2, USP15, HOXC8, ZNF423, ZNF521, SKOR1, LEMD3, PSMB4, OAZ1, FAM83G, ZC3H3, TMEM119, ZNF8, RANBP3L, EGR1, SMAD6, and YAP1 highlight the complex and dynamic nature of SMAD1's regulatory network, emphasizing its central role in cellular processes.

Q15797;O15198

Observed band size: 58-60 kDa

Protein G

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat, Monkey

WB: 1:500-2000; ICC/IF: 1:100-500; ELISA: 1:1000-5000

WB, ICC/IF, ELISA

Liquid

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2a

Synthesized peptide derived from human Smad1/9 AA range: 400-465