Skp2 Antibody (YA5059)

Skp2 Antibody (YA5059) is a mouse-derived and non-conjugated monoclonal antibody, targeting to Skp2. It can be applicated for WB, ELISA assays, in the background of human.

SKP2 serves as the substrate recognition component within the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex, orchestrating the ubiquitination and subsequent proteasomal degradation of target proteins pivotal in cell cycle progression, signal transduction, and transcription. It specifically recognizes phosphorylated CDKN1B/p27kip, playing a crucial role in the regulation of the G1/S transition. SKP2's substrate spectrum encompasses proteins like ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, YTHDF2, MYC, TOB1, and TAL1, among others. Additionally, SKP2 is involved in the ubiquitin-mediated proteasomal degradation of hepatitis C virus non-structural protein 5A, displaying antiviral activity against the virus. The complex nature of SKP2's interactions underscores its significance in governing diverse cellular processes through targeted protein degradation.

Q13309

Predicted band size: 48 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:2000; ELISA: 1:10000

WB, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of Skp2 p45 (aa1-130) expressed in E. Coli.