SIRT6 Antibody (YA5214)

SIRT6 Antibody (YA5214) is a mouse-derived and non-conjugated monoclonal antibody, targeting to SIRT6. It can be applicated for WB, ICC/IF, IP assays, in the background of human, mouse, rat, monkey.

SIRT6 is an NAD-dependent protein deacetylase, deacylase, and mono-ADP-ribosyltransferase that plays pivotal roles in DNA damage repair, telomere maintenance, metabolic homeostasis, inflammation, tumorigenesis, and aging. It catalyzes deacetylation of histone H3 at Lys-9, Lys-18, and Lys-56 (H3K9ac, H3K18ac, and H3K56ac), suppressing target gene expression of transcription factors like NF-kappa-B. SIRT6 participates in double-strand break (DSB) repair by binding DSB sites, recruiting repair proteins (e.g., SMARCA5/SNF2H), and deacetylating H3K9ac/H3K56ac (By similar mechanisms potentially linked to lifespan extension). It also promotes DNA repair via deacetylation of non-histone proteins (e.g., DDB2 and p53/TP53). Metabolically, SIRT6 inhibits glycolysis (by repressing HIF1A), regulates gluconeogenesis (via FOXO1/KAT2A deacetylation), and modulates lipid catabolism (by activating PPARA through NCOA2 deacetylation) (By similar roles in brown adipocytes). Its defatty-acylase activity (e.g., demyristoylation/depalmitoylation of RRAS2/TNF) controls protein secretion, while mono-ADP-ribosylation (e.g., of PARP1/TRIM28) contributes to DNA repair and transcriptional regulation.

Q8N6T7

Observed band size: 42 kDa

affinity chromatography.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat, Monkey

WB: 1:500; IF/ICC1:100

WB, ICC/IF, IP

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant human SIRT6 protein expressed in E.coli.