SIRT1 Antibody (YA082)

SIRT1 Antibody (YA082) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SIRT1.

SIRT1, a NAD-dependent protein deacetylase, orchestrates the integration of various cellular functions, including cell cycle progression, response to DNA damage, metabolism, apoptosis, and autophagy, by modulating the acetylation status of numerous target proteins. Functioning as a sensor of the NAD(+)/NADH ratio, SIRT1 responds to changes in cellular energetics induced by factors such as glucose deprivation and caloric restriction. Its wide-ranging impact encompasses chromatin remodeling through histone deacetylation, ultimately leading to transcriptional regulation. SIRT1 engages in diverse interactions, deacetylating transcription factors, coregulators, and histones, thereby exerting both positive and negative regulation on target gene expression. Additionally, SIRT1 is implicated in critical processes such as DNA damage response, autophagy, circadian rhythm regulation, and metabolic homeostasis. Its multifaceted roles extend to the regulation of various cellular pathways and the maintenance of genomic integrity, highlighting its significance in cellular physiology and stress response.

Free

Q96EB6

Predicted band size: 82 kDa;Observed band size: 110-130 kDa

Protein A affinity purified.

Nucleus, Cytoplasm, Mitochondrion.

Non-conjugated

Unmodified

AB_3102425

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:1000; ChIP: Use 0.5~2 μg for 25 μg of chromatin.

• 
  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using SIRT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80319, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
• 
  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using SIRT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80319, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
• 
  Immunohistochemical analysis of paraffin-embedded human testis tissue using SIRT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80319, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
• 
  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using SIRT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80319, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

WB, IHC-P, ICC/IF, ChIP

HeLa cell lysate, HEK-293 cell lysate, A549 cell lysate, F9 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate,

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human SIRT1.AA range:698-747.