Shh Antibody

Shh Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Shh.

The sonic hedgehog (SHH) protein precursor undergoes autoproteolysis and cholesterol transferase activity in its C-terminal part, leading to the cleavage of the full-length protein into two fragments, ShhN and ShhC, with the covalent attachment of a cholesterol moiety to the C-terminus of the newly generated ShhN. These processes occur in the endoplasmic reticulum, where ShhC is subsequently degraded. The dually lipidated sonic hedgehog protein N-product (ShhNp) serves as a morphogen essential for diverse patterning events in development. ShhNp induces ventral cell fate in the neural tube and somites, participates in anterior-posterior axis patterning in the developing limb bud, and plays a crucial role in axon guidance. SHH binds to the patched (PTCH1) receptor, working in conjunction with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO.

Q15465

Predicted band size: 19/47 kDa; Observed band size: 49 kDa

affinity purified

Sonic hedgehog protein C-product: Secreted, extracellular space. Note=The C-terminal peptide diffuses from the cell. Sonic hedgehog protein N-product: Cell membrane; Lipid-anchor. Note=The N-product either remains associated with lipid rafts at the cell surface, or forms freely diffusible active multimers with its hydrophobic lipid-modified N- and C-termini buried inside.

Non-conjugated

Unmodified

AB_3103077

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

Chicken, Cow, Horse, Rabbit

WB: 1:500-2000;ELISA: 1:5000-10000;IHC-P: 1:100-500;IHC-F: 1:100-500;IF: 1:100-500

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  Western blot analysis of extracts from C2C12 using Shh antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ELISA, IHC-P, IHC-F, ICC/IF

Liquid

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from human Shh: 21-120/462