Shh Antibody (YA5058)

Shh Antibody (YA5058) is a mouse-derived and non-conjugated monoclonal antibody, targeting to Shh. It can be applicated for WB, ELISA assays, in the background of human.

The sonic hedgehog (SHH) protein precursor undergoes autoproteolysis and cholesterol transferase activity in its C-terminal part, leading to the cleavage of the full-length protein into two fragments, ShhN and ShhC, with the covalent attachment of a cholesterol moiety to the C-terminus of the newly generated ShhN. These processes occur in the endoplasmic reticulum, where ShhC is subsequently degraded. The dually lipidated sonic hedgehog protein N-product (ShhNp) serves as a morphogen essential for diverse patterning events in development. ShhNp induces ventral cell fate in the neural tube and somites, participates in anterior-posterior axis patterning in the developing limb bud, and plays a crucial role in axon guidance. SHH binds to the patched (PTCH1) receptor, working in conjunction with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO.

Q15465

Predicted band size: 50 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:2000; ELISA: 1:10000

WB, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of human Shh expressed in E. Coli.