SETDB1/ESET Antibody (YA4277)

SETDB1/ESET Antibody (YA4277) is a mouse-derived and non-conjugated IgG1 antibody, targeting to SETDB1. It can be applicated for WB, ICC/IF, ELISA assays, in the background of human, mouse, monkey.

ESET is a histone methyltransferase that specifically trimethylates histone H3 at lysine 9 (H3K9me3), a mark for epigenetic transcriptional repression via recruitment of HP1 proteins (e.g., CBX1, CBX3, and/or CBX5). It primarily functions in euchromatin, playing a central role in silencing euchromatic genes, and coordinates with DNA methylation (e.g., in HUSH-mediated heterochromatin formation). ESET forms a complex with MBD1 and ATF7IP to repress transcription by coupling DNA methylation and H3K9me3, with its activity heritably maintained by CAF-1 during DNA replication. TRIM28/TIF1B recruits ESET to H3, facilitated by KRAB zinc-finger proteins. In colorectal cancer cells, ESET likely mediates KRAS-activated promoter hypermethylation and silencing of tumor suppressor genes (TSGs). It also maintains repressive gene states in undifferentiated embryonic stem cells (ESCs) and, with TRIM28, silences endogenous/retroviral elements independently of DNA methylation (by similar). The ESET-TRIM28-ZNF274 complex may guide ATRX to zinc-finger gene exons to safeguard H3K9me3 at transcriptionally active regions.

Q15047

Predicted band size: 143 kDa; Observed band size: 180 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse, Monkey

WB: 1/500-1/2000; IF: 1/200-1/1000; ELISA: 1/10000

WB, ICC/IF, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human SETDB1 expressed in E. Coli.