SAMHD1 Antibody (YA1542)

SAMHD1 Antibody (YA1542) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SAMHD1.

The related gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in The related gene have been associated with Aicardi-Goutieres syndrome.

Q9Y3Z3

Predicted band size: 72 kDa; Observed band size: 72 kDa

Affinity Chromatography

Non-conjugated

Unmodified

Microbiology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; FC: 1:50-1:100

• 
  Western blot analysis was performed on extracts from K562 (lane 1, 15 μg), Hela (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), and A549 (lane 4, 15 μg) using SAMHD1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P, ICC/IF, FC

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human SAMHD1