RYK Antibody (YA2481)

RYK Antibody (YA2481) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2481), targeting RYK, with a predicted molecular weight of 68 kDa (observed band size: 72 kDa). RYK Antibody (YA2481) can be used for WB, IHC-F, IHC-P, ICC/IF, IP experiment in human background.

RYK Protein appears to function as a potential coreceptor, collaborating with FZD8, for Wnt proteins like WNT1, WNT3, WNT3A, and WNT5A. Its involvement spans critical processes such as neuron differentiation, axon guidance, corpus callosum establishment, and neurite outgrowth, emphasizing its significance in neural development. Upon stimulation by WNT3, the receptor undergoes C-terminal cleavage in its transmembrane region, facilitating the translocation of the C-terminal intracellular product from the cytoplasm to the nucleus. This translocated product assumes a crucial role in mediating neuronal development. The multifaceted engagement of RYK in Wnt signaling pathways underscores its potential as a key player in orchestrating intricate cellular processes integral to neural differentiation and growth. Further exploration of RYK's functions and regulatory mechanisms may provide deeper insights into its specific contributions to neuronal development.

P34925

Predicted band size: 68 kDa; Observed band size: 72 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3104834

-

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200;IP: 1/20

• 
  Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), HeLa (lane 2, 20 μg), A549 (lane 3, 20 μg), and U2OS (lane 4, 20 μg) using RYK Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-F, IHC-P, ICC/IF, IP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthetic peptide of human RYK