RUNX3 Antibody (YA4253)

RUNX3 Antibody (YA4253) is a mouse-derived and non-conjugated IgG2b antibody, targeting to RUNX3. It can be applicated for IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse.

RUNX3 forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX family members modulate transcription by recognizing the core binding sequence 5'-TGTGGT-3' or, rarely, 5'-TGCGGT-3' in regulatory regions via their runt domain, while CBFB enhances RUNX's DNA-binding capacity allosterically. The heterodimer binds to core sites in enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3, and GM-CSF promoters (By similarity). RUNX3 may regulate cellular proliferation and/or differentiation. In association with ZFHX3, it upregulates CDKN1A promoter activity following TGF-beta stimulation. CBF complexes repress ZBTB7B during cytotoxic (CD8+) T cell development by binding to RUNX-binding sequences in the ZBTB7B locus, acting as a transcriptional silencer (By similarity). Additionally, RUNX3 is essential for the development and survival of parvalbumin-expressing sensory neurons (By similarity).

Q13761

Predicted band size: 44 kDa; Observed band size: 44 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse

IHC: 1/200-1/1000; IF: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Purified recombinant fragment of human RUNX3 (AA:186-252) expressed in E. Coli.