2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. ROCK1 Antibody (YA089)

ROCK1 Antibody (YA089) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ROCK1.

For research use only. We do not sell to patients.

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50 μL In-stock
100 μL In-stock
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Top Publications Citing Use of Products

Publications Citing Use of MCE ROCK1 Antibody (YA089)

【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

ROCK1 Antibody (YA089) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ROCK1.
Background

ROCK1 protein stands as a pivotal kinase, wielding key regulatory influence over the actin cytoskeleton and cellular polarity. Demonstrating a broad spectrum of cellular functions, ROCK1 intricately governs smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion, and motility through the phosphorylation of diverse substrates such as DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1, and PPP1R12A. It collaborates with FHOD1 to induce SRC-dependent non-apoptotic plasma membrane blebbing and phosphorylates JIP3, regulating the recruitment of JNK to JIP3 during UVB-induced stress. As a suppressor of inflammatory cell migration, ROCK1 influences PTEN phosphorylation and stability, while concurrently acting as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Crucially involved in centrosome positioning and mitotic exit, ROCK1 plays roles in terminal erythroid differentiation, inhibition of podocyte motility, promotion of keratinocyte terminal differentiation, and facilitation of osteoblast compaction during matrix assembly, contributing to osteoblast mineralization. Moreover, ROCK1 may impact eyelid and ventral body wall closure through the induction of actomyosin bundle assembly. The diverse roles of ROCK1 underscore its multifaceted impact on cellular physiology and highlight its regulatory significance in various cellular processes.
Tag

Free
Gene ID
SwissProt ID
Molecular Weight

Predicted band size: 158 kDa; Observed band size: 158 kDa
Purity

Protein A affinity purified.
Subcellular Location

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane.
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Signal Transduction
Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
Clonality

Recombinant, Monoclonal
Host

Rabbit
Reactivity

Human, Mouse
Dilution Ratio

WB: 1:500-1:5000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200; IP: Use at an assay dependent concentration.
Application

WB, ICC/IF, IHC-P, IP
Appearance

Liquid
Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

Synthetic peptide corresponding to Human ROCK1.AA range:1104-1149.
Database

Entrez Gene: 6093 Human ; 19877 Mouse ;

SwissProt: Q13464 Human ; P70335 Mouse ; Q63644 Rat

OMIM: 601702 Human

Documentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

ROCK1 Antibody (YA089) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
ROCK1 Antibody (YA089)
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HY-P80314
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