Rictor Antibody (YA5045)

Rictor Antibody (YA5045) is a mouse-derived and non-conjugated monoclonal antibody, targeting to Rictor. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse, monkey.

RICTOR is a core component of the mechanistic target of rapamycin complex 2 (mTORC2), which relays growth factor signals to pathways governing proliferation, cytoskeletal organization, lipogenesis, and anabolic metabolism. mTORC2 phosphorylates and activates AGC kinase family members (e.g., AKT, PKC, and SGK1) and, unlike mTORC1, is insensitive to nutrient availability. RICTOR likely functions as a molecular scaffold within mTORC2 and confers FKBP12-rapamycin insensitivity. The complex mediates context-dependent phosphorylation of AKT1 (e.g., at Thr-450, Ser-473) for full activation, and phosphorylates SGK1 (Ser-422) and PRKCA (Ser-657) (by similar). It also regulates insulin signaling (e.g., via FBXW8 and IGF2BP1 phosphorylation) (by similar) and modulates actin cytoskeleton dynamics through Rho GTPases.

Q6R327

Predicted band size: 192 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Monkey

WB: 1:500-1:2000; IHC: 1:200-1:1000; ICC/IF: 1:200-1:1000; FC: 1:200-1:400; ELISA: 1:10000

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of human Rictor expressed in E. Coli.