RhoA Antibody (YA4122)

RhoA Antibody (YA4122) is a mouse-derived and non-conjugated IgG2b antibody, targeting to RHOA. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human.

RhoA Protein is a small GTPase crucial for orchestrating diverse cellular responses, cycling between an active GTP-bound state and an inactive GDP-bound state. Primarily associated with the organization of the cytoskeleton, the active form of RhoA binds to various effector proteins, influencing cellular processes such as cytoskeletal dynamics, cell migration, and cell cycle progression. It plays a pivotal role in regulating signal transduction pathways that connect plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Additionally, RhoA is indispensable for microtubule-dependent signals required for myosin contractile ring formation during cell cycle cytokinesis, contributing to cleavage furrow formation. Essential for apical junction formation in keratinocyte cell-cell adhesion, RhoA also participates in the MEMO1-RHOA-DIAPH1 signaling pathway, influencing ERBB2-dependent microtubule stabilization. It further modulates potassium channel activity, regulates guanine nucleotide exchange factors, and acts as a target for the yopT cysteine peptidase from Yersinia pestis during microbial infection.

P61586

Predicted band size: 22 kDa; Observed band size: 22 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human

WB: 1/500-1/2000; IHC: 1/200-1/1000; IF: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2b

Purified recombinant fragment of human RHOA (AA: 1-190) expressed in E. Coli.