RF1 Antibody (YA4125)

Cat. No.: HY-P84428: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

RF1 Antibody (YA4125) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RF1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

Biological Activity
Technical Parameters
Properties
Documentation

Description	RF1 Antibody (YA4125) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RF1.
Background	RF1 is a component of the eRF1-eRF3-GTP ternary complex, which mediates translation termination in response to stop codons. The complex binds to a stop codon in the ribosomal A-site, with ETF1/ERF1 responsible for stop codon recognition and peptidyl-tRNA hydrolysis. Following GTP hydrolysis, eRF3 dissociates, allowing ETF1/eRF1 to fully occupy the A-site and facilitate peptidyl-tRNA hydrolysis. RF1 is also part of the transient SURF complex, recruiting UPF1 to stalled ribosomes during nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Additionally, RF1 is required for SHFL-mediated translation termination, which inhibits programmed ribosomal frameshifting (-1PRF) in viral and cellular mRNAs. by similar: Functional mechanisms of the eRF1-eRF3-GTP complex and its role in translation termination.
Gene ID	2107 [NCBI]
SwissProt ID	P62495
Synonyms	ERF; ETF1; ERF1; TB3-1; D5S1995; SUP45L1
Molecular Weight	Predicted band size: 49 kDa; Observed band size: 49 kDa
Purity	affinity purified.
Conjugation	Non-conjugated
Product Categories	Primary Antibody; Mouse Monoclonal Antibody
Clonality	Monoclonal Antibody
Host	Mouse
Reactivity	Human
Dilution Ratio	WB: 1:500-1:2000; FC: 1:200-1:400; ELISA: 1:10000
Application	WB, FC, ELISA
Appearance	Liquid
Formulation	Supplied in PBS with 0.05% sodium azide
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG1
Immunogen	Purified recombinant fragment of human RF1 (AA: 288-437) expressed in E. Coli.
Documentation	Select Batch: Data Sheet (234 KB) SDS (251 KB) COA (205 KB) User Guide for Antibodies (1077 KB)

RF1 Antibody (YA4125) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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RF1 Antibody (YA4125)

Biological Activity

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Documentation

RF1 Antibody (YA4125) Related Classifications

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