RF1 Antibody (YA4125)

Cat. No.: HY-P84428: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

RF1 Antibody (YA4125) is a mouse-derived and non-conjugated IgG1 antibody, targeting to RF1. It can be applicated for WB, FC, ELISA assays, in the background of human.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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【WB: Western Blot; IHC-P: Immunohistochemistry-Paraffin; IHC-F: Immunohistochemistry-Frozen; ICC/IF: Immunocytochemistry/Immunofluorescence; IF-Tissue: Immunofluorescence-Tissue; mIHC: Multiplex Immunohistochemical; IP: Immunoprecipitation; ChIP: Chromatin Immunoprecipitation; FC: Flow Cytometry; ELISA: Enzyme Linked Immunosorbent Assay】

Biological Activity
Technical Parameters
Properties
Documentation

Description	RF1 Antibody (YA4125) is a mouse-derived and non-conjugated IgG1 antibody, targeting to RF1. It can be applicated for WB, FC, ELISA assays, in the background of human.
Background	RF1 is a component of the eRF1-eRF3-GTP ternary complex, which mediates translation termination in response to stop codons. The complex binds to a stop codon in the ribosomal A-site, with ETF1/ERF1 responsible for stop codon recognition and peptidyl-tRNA hydrolysis. Following GTP hydrolysis, eRF3 dissociates, allowing ETF1/eRF1 to fully occupy the A-site and facilitate peptidyl-tRNA hydrolysis. RF1 is also part of the transient SURF complex, recruiting UPF1 to stalled ribosomes during nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Additionally, RF1 is required for SHFL-mediated translation termination, which inhibits programmed ribosomal frameshifting (-1PRF) in viral and cellular mRNAs. by similar: Functional mechanisms of the eRF1-eRF3-GTP complex and its role in translation termination.
Gene ID	2107 [NCBI]
SwissProt ID	P62495
Synonyms	ERF; ETF1; ERF1; TB3-1; D5S1995; SUP45L1
Molecular Weight	Predicted band size: 49 kDa; Observed band size: 49 kDa
Purity	affinity purified.
Conjugation	Non-conjugated
Product Categories	Primary Antibody; Mouse Monoclonal Antibody
Host	Mouse
Reactivity	Human
Dilution Ratio	WB: 1/500-1/2000; FC: 1/200-1/400; ELISA: 1/10000
Application	WB, FC, ELISA
Appearance	Liquid
Formulation	Supplied in PBS with 0.05% sodium azide
Storage & Stability	Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping	Shipping with blue ice.
Isotype	IgG1
Immunogen	Purified recombinant fragment of human RF1 (AA: 288-437) expressed in E. Coli.
Documentation	Select Batch: Data Sheet (234 KB) SDS (251 KB) COA (205 KB) User Guide for Antibodies (1077 KB)

RF1 Antibody (YA4125) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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RF1 Antibody (YA4125)

Biological Activity

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Properties

Documentation

RF1 Antibody (YA4125) Related Classifications

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