Renilla Luciferase Antibody (YA3013)

Renilla Luciferase Antibody (YA3013) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3013), targeting Renilla Luciferase, with a predicted molecular weight of 36 kDa (observed band size: 36 kDa). Renilla Luciferase Antibody (YA3013) can be used for WB, ICC/IF experiment in renilla reniformis background.

Renilla Luciferase is an enzyme that, upon binding its substrate, catalyzes an oxygenation reaction, producing a highly unstable hydroperoxide that cyclizes into a dioxetanone structure. The dioxetanone then collapses, releasing a CO(2) molecule. The spontaneous breakdown of the dioxetanone releases approximately 50 kcal/mol of energy, which is required to generate the excited state of the coelenteramide product (the singlet form of the monoanion). In vivo, the product undergoes nonradiative energy transfer to an accessory protein, green fluorescent protein (GFP), resulting in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.

P27652

Predicted band size: 36 kDa; Observed band size: 36 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3105382

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Renilla reniformis

WB: 1/500-1/1000;IF: 1/50-1/200

WB, ICC/IF

Liquid

Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from Renilla Luciferase