RALA Antibody (YA3752)

RALA Antibody (YA3752) is a mouse-derived and non-conjugated IgG1 antibody, targeting to RALA. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse, rat, monkey.

RALA is a multifunctional GTPase involved in diverse cellular processes such as gene expression, cell migration, cell proliferation, oncogenic transformation, and membrane trafficking. It achieves these functions by interacting with distinct downstream effectors (e.g., the exocyst complex, GRK2, and RALBP1). RALA acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles and regulates integrin-dependent membrane raft exocytosis and growth signaling via the RALA-exocyst complex. It is also a key regulator of LPAR1 signaling, competing with GRK2 for LPAR1 binding to modulate receptor signaling properties. During mitosis, RALA stabilizes and elongates the intracellular bridge between dividing cells and cooperates with EXOC2 to recruit exocyst components to the early midbody. Additionally, RALA controls mitochondrial fission by recruiting RALBP1 to mitochondria, where RALBP1 facilitates DNM1L phosphorylation and activation by the mitotic kinase cyclin B-CDK1.

P11233

Predicted band size: 24 kDa; Observed band size: 25 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse, Rat, Monkey

WB: 1/500-1/2000; IHC: 1/100-1/500; IF: 1/50-1/500; FC: 1/200-1/400; ELISA: 1/10000

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human RALA (AA: 71-203) expressed in E. Coli.