RAC1/2/3 Antibody (YA3290)

RAC1/2/3 Antibody (YA3290) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAC1/2/3.

The RAC1 Protein, a plasma membrane-associated small GTPase, dynamically cycles between its active GTP-bound and inactive GDP-bound states, exerting regulatory control over a spectrum of cellular responses. In its active state, RAC1 binds to various effector proteins, modulating processes such as secretory functions, phagocytosis of apoptotic cells, epithelial cell polarization, neuronal adhesion, migration, differentiation, and growth-factor-induced formation of membrane ruffles. It forms an active heterodimer with Rho GDI in the cytosol, known as sigma 1, participating in the stimulation of NADPH oxidase activity in macrophages. Essential for SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly, RAC1 also stimulates PKN2 kinase activity. It collaborates with RAB7A to regulate the formation of ruffled borders in osteoclasts and promotes the nuclear shuttling of NR3C2 in podocytes for proper kidney functioning. In neurons, RAC1 is involved in dendritic spine formation, synaptic plasticity, spine morphogenesis, and synapse formation, highlighting its multifaceted role in neurobiology. Furthermore, RAC1 plays a crucial role in regulating GABA(A) receptor synaptic stability and GABAergic inhibitory synaptic transmission through PAK1 activation and F-actin stabilization. The isoform B of RAC1 exhibits distinctive features in its GEF-independent GDP/GTP exchange and impaired GTP hydrolysis, partially restored by GTPase-activating proteins, suggesting nuanced regulatory mechanisms in its effector interactions.

P63000 / P15153 / P60763

Predicted band size: 21 kDa; Observed band size: 21 kDa

Affinity Chromatography

Non-conjugated

Unmodified

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1/500-1/1000

• 
  Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), Hela (lane 2, 15 μg), HUVEC (lane 3, 15 μg), C6 (lane 4, 15 μg), RAW264.7 (lane 5, 15 μg), and 3T3 (lane 6, 15 μg) using Rac Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB

Liquid

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human Rac1/2/3