PYK2 Antibody (YA5033)

PYK2 Antibody (YA5033) is a mouse-derived and non-conjugated monoclonal antibody, targeting to PYK2. It can be applicated for WB, IHC-P, ICC/IF, ELISA assays, in the background of human.

PYK2 is a non-receptor protein-tyrosine kinase that orchestrates actin cytoskeleton reorganization, cell polarization, migration, adhesion, spreading, and bone remodeling. It modulates humoral immunity by maintaining marginal zone B-cell populations in the spleen and enabling B-cell trafficking, while being essential for macrophage polarization and chemotaxis toward inflammatory sites. In T-cells, it regulates cytoskeletal rearrangement and contributes to immune responses. Collaborating with SRC, it enhances osteoclastic bone resorption and may suppress osteoprogenitor differentiation. Functioning downstream of diverse receptors (integrins, GPCRs, cytokine/chemokine receptors) and stress signals, it forms activated signaling complexes with SRC family kinases to create phosphotyrosine docking sites. Its multifaceted signaling includes PI3K-AKT1 activation, NOS3 stimulation, cGMP production, MAPK cascade initiation (ERK1/2, JNK1), and Rho GTPase (RHOA/RAC1) regulation. Nuclear PYK2 recruits MDM2 to modulate p53 ubiquitination/degradation, and acts as a scaffold for SRC-mediated PDPK1 phosphorylation. It influences NMDA receptor activity via SRC-family phosphorylation, potentially regulates potassium channels, and auto-amplifies SRC kinase activity. Substrates include ASAP1, NPHP1, KCNA2, SHC1, with SRC-cooperative phosphorylation of ASAP2, RHOU, and PXN.

Q14289

Predicted band size: 116 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:2000; IHC: 1:200-1:1000; ELISA: 1:10000; ICC/IF: 1:50-200

WB, IHC-P, ICC/IF, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of PYK2 (aa815-997) expressed in E. Coli.