PTEN Antibody (YA4114)

PTEN Antibody (YA4114) is a mouse-derived and non-conjugated IgG1 antibody, targeting to PTEN. It can be applicated for WB, ICC/IF, FC, ELISA assays, in the background of human, mouse.

PTEN is a dual-specificity protein phosphatase that dephosphorylates tyrosine-, serine-, and threonine-phosphorylated proteins. It also functions as a lipid phosphatase, preferentially acting on PtdIns(3,4,5)P3/phosphatidylinositol 3,4,5-trisphosphate to remove the phosphate group at the D3 position of the inositol ring. Additionally, PTEN acts as a cytosolic inositol 3-phosphatase, targeting Ins(1,3,4,5,6)P5/inositol 1,3,4,5,6 pentakisphosphate and possibly Ins(1,3,4,5)P4/1D-myo-inositol 1,3,4,5-tetrakisphosphate. By dephosphorylating phosphoinositides, PTEN antagonizes the PI3K-AKT/PKB signaling pathway, modulating cell cycle progression and survival. In motile cells, it suppresses lateral pseudopod formation, promoting polarization and directed movement. PTEN also dephosphorylates tyrosine-phosphorylated focal adhesion kinase, inhibiting migration, integrin-mediated spreading, and focal adhesion formation. It plays a key role in growth factor-induced epithelial cell migration, interacting with DLC1 or p85 to regulate RHOA and RAC1 activation. Furthermore, PTEN is a critical modulator of the AKT-mTOR pathway, influencing neuronal integration during adult neurogenesis (by similar). In excitatory hippocampal synapses, PTEN regulates synaptic function and plasticity, with its lipid phosphatase activity driving AMPA receptor-mediated synaptic depression, essential for NMDA receptor-dependent long-term depression (LTD) (by similar). PTEN may negatively regulate insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form exhibits stronger pro-apoptotic potential, while the cytoplasmic nonubiquitinated form has weaker tumor-suppressive activity.

P60484

Predicted band size: 47 kDa; Observed band size: 54 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Mouse

WB: 1/500-1/2000; IF: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

WB, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of PTEN expressed in E. Coli.