PSAP Antibody (YA3690)

PSAP Antibody (YA3690) is a mouse-derived and non-conjugated IgG1 antibody, targeting to PSAP. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human, rat.

PSAP is a low-molecular-mass non-enzymatic protein involved in the lysosomal degradation of sphingolipids. Specifically, saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by β-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by β-galactosylceramidase (EC 3.2.1.46), with saposin-C forming an activated complex with the enzyme and acidic lipids rather than solubilizing the substrate. Saposin-B promotes the degradation of galacto-cerebroside sulfate (via arylsulfatase A, EC 3.1.6.8), GM1 gangliosides (via β-galactosidase, EC 3.2.1.23), and globotriaosylceramide (via α-galactosidase A, EC 3.2.1.22) by forming substrate-solubilizing complexes. Saposin-D acts as a specific activator of sphingomyelin phosphodiesterase (EC 3.1.4.12). Additionally, PSAP exhibits myelinotrophic and neurotrophic effects mediated by G-protein-coupled receptors GPR37 and GPR37L1, leading to ligand internalization and ERK phosphorylation signaling. by similar: Mechanisms of sphingolipid-metabolizing enzyme activation and receptor-mediated signaling pathways.

P07602

Predicted band size: 58 kDa; Observed band size: 70 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Mouse

Human, Rat

WB: 1/500-1/2000; IHC: 1/200-1/1000; IF: 1/200-1/1000; FC: 1/200-1/400; ELISA: 1/10000

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS with 0.05% sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human PSAP (AA: 17-216) expressed in E. Coli.