Prohibitin Antibody (YA5028)

Prohibitin Antibody (YA5028) is a mouse-derived and non-conjugated monoclonal antibody, targeting to Prohibitin. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human, mouse, rat, monkey.

PHB is a protein with pleiotropic functions mediated in a cell-compartment- and tissue-specific manner. At the plasma membrane, it cooperates with CD86 to mediate CD86-signaling in B lymphocytes, regulating IgG1 production (By similarity). Upon CD40 engagement, PHB activates the NF-κB signaling pathway via phospholipase C and protein kinase C (By similarity). In mitochondria, PHB forms large ring complexes (prohibitin complexes) with PHB2, functioning as a chaperone to stabilize mitochondrial respiratory enzymes and maintain inner mitochondrial membrane integrity, crucial for mitochondrial morphogenesis, neuronal survival, and lifespan (Probable). The prohibitin complex, along with DNAJC19, regulates cardiolipin remodeling and OMA1 protein turnover (By similarity). PHB also contributes to mitochondrial-mediated antiviral innate immunity by activating RIG-I signaling and promoting IFNB1 and pro-inflammatory cytokine IL6 production. In the nucleus, PHB acts as a transcriptional co-regulator, enhancing TP53 promoter binding (activating transcription) while reducing E2F1 promoter binding (repressing transcription). Additionally, PHB interacts with STAT3 to modulate IL17 secretion in Th17 cells.

P35232

Predicted band size: 30 kDa;

affinity purified.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat, Monkey

WB: 1:500-1:2000; IHC: 1:200-1:1000; ICC/IF: 1:200-1:1000; FC: 1:200-1:400; ELISA: 1:10000

WB, IHC-P, ICC/IF, FC, ELISA

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant fragment of human Prohibitin expressed in E. Coli.